Specimen Collection Protocols:
Protect sample from light
CSF should be drawn at least 12 hours after a suspected event.
Sample must be delivered to lab with in 1 hr
Aliquot Instructions: CSF 500 uL Fridge - protect from light, Last aliquot drawn to be forwarded for analysis, Centrifuge sample within one hour, send supernatant for analysis. Send ASAP, fridge temperature
Xanthochromia may be indicative of: cerebral haemorrhage; cerebral tumor; impaired circulation of the CSF; jaundice – a rise in unconjugated bilirubin may impart a yellow colour to CSF.
Initail screening is by measurement of CSF Bilirubin. Levels above 335 nmol/L are confirmed spectrophotometrically.Reference; Revised national Guidelines for anaylysis of CSF Fluid for Bilirubin in suspected SAH. Ann. Clin. Biochem 2008;45.. 238-244
CSF is scanned using a spectrophotometer from 400-700 nm to detect absorption peaks for the presence of breakdown products of haemoglobin. This is a qualitative test.
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