A 5 ml non gel heparin tube is the prefered sample type.
If other biochemistry is requested requiring a heparin specimen, please collect a separate tube for the vitamin B1
2 mL whole blood, to be sent to Specialist lab urgently, via hoist (phone 80332). After hours prepare in Separating area as per protocol. Wash with NaCL, dil cells1/1with dist H2O and freeze.
Send frozen prepared haemolysed cells
Recommended Range >140 nmol/L
HPLC following production of fluorescent product
$68.02 (Exclusive of GST)
The red blood cells must be washed, haemolysed and frozen before they are 4 hours old (post venipuncture). Samples for analysis of thiamine are prepared by washing the red cells with isotonic 0.9% saline, preparing a red cell lysate and freezing.
1.Centrifuge the sample at 3000 rpm for 5 minutes.
2.After centrifugation use a pasteur pipette to remove and discard the plasma and buffy coat layer.
3.The red cells are washed by adding an equivalent volume of isotonic saline (9 g/L NaCl) and mixing thoroughly.
4.Centrifuge the sample at 3000 rpm for 5 min.
5.Label a plastic tube suitable for transport, with the patient surname and laboratory number.
6.After centrifugation use a pasteur pipette to remove the top saline layer and 1 mm of red cells. Discard, leaving behind the washed red cells.
7.To the labelled tube add 1 mL of washed packed red blood cells (RBC) from the middle of the washed cells.
8.Using the same pipette tip, draw up 1 mL of distilled water and add this to the cells in the labelled tube.
9.Stopper, mix thoroughly by rolling the haemolysate around the inside of the tube.
10.Place the tube inside a plastic bag and freeze.
When frozen, send to laboratory.