URGENT GBM / ANCA Screen only

Diagnostic Use

For urgent serological testing in the context of new presentation of ANCA-associated vasculitis and/or anti-GBM disease, usually associated with rapid renal failure and/or a pulmonary-renal syndrome (haemoptysis)
Rapid testing for anti-MPO, anti-PR3 and anti-GBM for same day results Mon-Fri, business hours- testing by arrangement only - the Immunology laboratory MUST be telephoned (x89787)

Department

Immunology

Delphic Registration Code

MP3, GBM

Laboratory Handling

Phlebotomy

5ml red, telephone to lab immediately

Registration

send direct to immunology

Separating

Separation by Immunology as required.

Synonyms

GBM/MPO/PR3 screen
Urgent GBM antibodies
Urgent Vasculitis screen
Vasculitis screen
GBAN

Turnaround Time

1 day

Test Code

7028, 7029, 6939

Specimen Type

• Serum

Specimen Requirements

Tube/Container Type:

Preferred

• Serum (Red Cap)

Preferred Sample Volume (ml)

5

No of Samples Required

1

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• Serum (Red Cap)

Preferred Sample Volume (ml)

0.5

Minimum Sample Volume (ml)

0.3

Aliquot Instructions

Sample Type

• Serum

Minimum Volume (ml)

0.2

Transport to Lab

Ambient (8 - 24 degrees Celsius)

Additional Referral/Aliquot Information

300 uL serum - minimum, 1 mL serum - preferred. Store in fridge

Interpretation

Elevated levels of either PR3-ANCA are highly predictive of a primary small vessel necrotizing vasculitis typically within the spectrum of Granulomatosis with polyangiitis (GPA) Microscopic polyangiitis (MPA) or an overlap syndrome. They may also be associated with drug induced Vasculitis, eg. D-penicillamine, Propylthiouracil and Minocycline associated Systemic Necrotising Vasculitis.

P-ANCA (of anti-MPO specificity) are found in up to 50 percent of patients with microscopic polyarteritis and pauci-immune glomerulonephritis, in addition to rheumatoid arthritis, SLE and ulcerative colitis.

Anti glomerular basement membrane antibodies are found in Goodpasture’s syndrome and levels correlate with disease activity and often predict clinical outcome. The autoantibody is directed against the NC1 domain of the α- chain of type IV collagen. Approximately 25% of patients presenting with anti GBM antibodies also present with anti-neutrophil cytoplasmic antibodies, so parallel testing is recommended.

Reference Intervals

For each antigen:

Negative <20 CU

Positive >20CU

Test Method

Chemiluminescence

Non-Funded Test Cost

$104.28 (Exclusive of GST)

Billing Code

6939

Additional Costing Information

Mon-Fri, business hours-urgent testing by arrangement only
Advance notice to the Immunology Laboratory is required.
Weekday call the laboratory on 03 364 0413.
This test is not performed during the weekend, any requests received over the weekend or on public holidays will be prioritized on the next working day.

Contact Information

Enquiry Form