Microbiology - Bacteriology
TB Polymerase Chain Reaction
If fresh tissue specimens are not available paraffin block specimens are acceptable. Please send 10-12 sections to the laboratory in a labelled specimen container at room temperature.. These are able to be tested by PCR only using AnyPlex method.
Polymerase Chain Reaction -
PCR for Mycobacterium tuberculosis is done by GeneXpert on request.
Sputum - will be decontaminated before PCR testing.
Pleural fluid - >5mLs undiluted or in EDTA tube.
Blood or bone marrow - Collect into EDTA tube.
FNA or tissue biopsy - Collect into sterile container.
CSF - Minimum 1.0 mL but preferably 5.0 mLs or more (consultation with Microbiologist desirable).
Seegene AnyPlex testing available for non-tuberculous mycobacteria after consultation with Microbiologist.
$222.80 (Exclusive of GST)
GeneXpert allows for detection of M.tuberculosis and determination of Rifampicin susceptibility from direct specimens within 24 - 48 hours of receipt in the laboratory. For M.tuberculosis on ZN positive respiratory specimens sensitivity and specificity is close to 100%. There is decreased sensitivity for ZN negative respiratory specimens and non-respiratory specimens.
Seegene AnyPlex allows for detection of MTB or other (non-speciated) mycobacteria.
Susceptibility testing, both genotypic and phenotypic, are completed when a M. tuberculosis culture has grown. First line susceptibilities include Rifampacin, Isoniazid, Ethambutol, Streptomycin and Pyrazinamde. For second Line susceptibilities please phone Microbiologist.
Not a routine test. Batched at least weekly, urgent testing after consultation with a Microbiologist only.