TB PCR

Department

Microbiology - Bacteriology

Delphic Registration Code

TBG

Synonyms

TB Polymerase Chain Reaction
Mycobacterium PCR
TB

Test Code

8240

Specimen Type

• Whole Blood
• Bone Marrow
• CSF
• Tissue
• Sputum
• Fluid

Specimen Requirements

Tube/Container Type:

Preferred

• Pottle

Acceptable

• EDTA (Lavender Cap)

Additional Specimen Information

If fresh tissue specimens are not available paraffin block specimens are acceptable. Please send 10-12 sections to the laboratory in a labelled specimen container at room temperature.. These are able to be tested by PCR only using AnyPlex method.

Aliquot Instructions

Test Method

Polymerase Chain Reaction -

PCR for Mycobacterium tuberculosis is done by GeneXpert on request.

Sputum - will be decontaminated before PCR testing.
Pleural fluid - >5mLs undiluted or in EDTA tube.
Blood or bone marrow - Collect into EDTA tube.
FNA or tissue biopsy - Collect into sterile container.
CSF - Minimum 1.0 mL but preferably 5.0 mLs or more (consultation with Microbiologist desirable).

Seegene AnyPlex testing available for non-tuberculous mycobacteria after consultation with Microbiologist.

Non-Funded Test Cost

$246.8 (Exclusive of GST)

Billing Code

8240

GeneXpert allows for detection of M.tuberculosis and determination of Rifampicin susceptibility from direct specimens within 24 - 48 hours of receipt in the laboratory. For M.tuberculosis on ZN positive respiratory specimens sensitivity and specificity is close to 100%. There is decreased sensitivity for ZN negative respiratory specimens and non-respiratory specimens.
Seegene AnyPlex allows for detection of MTB or other (non-speciated) mycobacteria. Up to 7 days.
Susceptibility testing, both genotypic and phenotypic, are completed when a M. tuberculosis culture has grown. First line susceptibilities include Rifampacin, Isoniazid, Ethambutol, Streptomycin and Pyrazinamde. For second Line susceptibilities please phone Microbiologist.

Not a routine test. Batched at least weekly, urgent testing after consultation with a Microbiologist only.

Contact Information

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