Detection or exclusion of T-cell clonal populations.
Genetics - Molecular Oncology
3x EDTA (Lavender) dedicated to Genetics
Send to MONC
T cell receptor gene rearrangement
12 ml peripheral blood in EDTA. 1-3 ml of bone marrow in EDTA or RPMI (anticoagulant).
5 mm cube of fresh (in saline or RPMI) or frozen tissue.
FFPET - block or at least 10 x 10 micron slices
Ambient (8 - 24 degrees Celsius)
Clonal populations of cells yield one (monoclonal), two (clonal) or more (oligoclonal) prominent amplicons within a specified size range. A normal (polyclonal) population of T cells will produce a polyclonal smear representing a heterogeneous population and, if graphed, would resemble a bell-shaped Gaussian curve.
Gene rearrangement and clonality are detected by PCR using primers for the V and J regions of the T-cell receptor gamma (TRG) gene.
Limit of Detection of Assay: ranges from 1 abnormal cell in 20 cells (in dense regions of the smear) to 1 abnormal cell in 100 cells (in less dense regions).
Paraffin-embedded tissue may, or may not, yield DNA of a suitable quality or quantity for this assay and may be reported as Insufficient.
Urgent testing by arrangement.
$412.1 (Exclusive of GST)
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