Detection or exclusion of T-cell clonal populations.
This test detects clonality to a level of 2.5% (5% of cells containing a heterozygous rearrangement).
Genetics - Molecular Oncology
2x EDTA (Lavender) dedicated to Genetics
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T cell receptor gene rearrangement
Clonality detection using this technique may be influenced by the T cell content of the sample and pseudoclonality may occur in samples with low T cell content.
4-8 ml peripheral blood in EDTA. 1-3 ml of bone marrow in EDTA or RPMI (anticoagulant).
5 mm cube of fresh (in saline or RPMI) or frozen tissue.
FFPET - block or at least 5 x 10 micron slices
Ambient (8 - 24 degrees Celsius)
Paraffin-embedded tissue may, or may not, yield DNA of a suitable quality or quantity for this assay and may be reported as insufficient.
Urgent testing is available by prior arrangement. Please contact the laboratory to request urgent testing on 03 378 6007.
Clonal populations of cells yield one (monoclonal), two (clonal) or more (oligoclonal) rearrangements. A normal population of T cells will produce a polyclonal pattern representing a heterogeneous population.
Clonality is determined if the most frequent rearrangement was present at 2.5% or greater and 2-fold that of the polyclonal background.
The sensitivity and specificity of this test compared to clinicopathologic information is ~85% and 92% respectively, as assessed by a small in-house verification (n = 26).
The T-cell receptor gamma (TRG) gene locus is analysed using multiplex PCR (Lymphotrack TRG assay, Invivoscribe Inc) and next generation sequencing on an Illumina MiSeq instrument. Secondary sequencing analysis is performed using Lymphotrack Dx software.
$602.00 (Exclusive of GST)
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