FISH can be used on paraffin embedded tissue sections to identify specific chromosome abnormalities.
FISH applied to formalin fixed paraffin embedded (FFPE) tissue is generally used for solid tumour specimens where viable tissue is not available for G-banding or routine FISH analysis.
Site-specific testing of tissues.
Testing for specific rearrangements where there is a differential diagnosis.
Confirmation of abnormalities that may be present in low proportions in bone marrow or solid tumour cultures due to loss of the malignant cells in culture.
Aid in the diagnosis of disease for tissue with poor histological morphology.
Genetics - Cytogenetics
Specimens are stable at room temperature at can be left until the next working day
Only tissue preserved in neutral buffered formalin and paraffin embedded is suitable for use.
NB: Samples de-calcified in formic acid are not suitable for FISH as this process affects the integrity of the DNA
Tissue specimens should be cut into sections of 2-4µm, on poly-L-lysine slides.
The H&E slide must be assessed by a pathologist prior to receipt and appropriate areas for FISH analysis clearly marked.
Ensure one H&E slide and 5~8 unprocessed slides are supplied.
Slide must be clearly labelled with two patient identifiers, matching those on the request form.
Ambient (8 - 24 degrees Celsius)
Transport in slide-holders or boxes, at ambient temperature
Clinically significant rearrangements and copy number changes outside of established normal ranges will be reported with relevant interpretive comment.
Fluorescent in situ hybridisation (FISH) on formalin fixed paraffin embedded (FFPE) tissue.
IGH/MYC gene fusion
IGH/BCL2 gene fusion
The same probes may be applied to a standard blood or bone marrow preparation if required. See 'Haematological and Oncological FISH'
$444.13 (Exclusive of GST)
The price listed is per probe set. Testing using multiple probes sets will be billed accordingly.
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