Solid Tumour FISH testing (FFPE)

Diagnostic Use

FISH can be used on paraffin embedded tissue sections to identify specific chromosome abnormalities.
FISH applied to formalin fixed paraffin embedded (FFPE) tissue is generally used for solid tumour specimens where viable tissue is not available for G-banding or routine FISH analysis.

FFPE-FISH allows:
Site-specific testing of tissues.
Testing for specific rearrangements where there is a differential diagnosis.
Confirmation of abnormalities that may be present in low proportions in bone marrow or solid tumour cultures due to loss of the malignant cells in culture.
Aid in the diagnosis of disease for tissue with poor histological morphology.

Department

Genetics - Cytogenetics

Delphic Registration Code

CTGN

Laboratory Handling

Registration

Specimens are stable at room temperature at can be left until the next working day

Synonyms

FFPE
MDM2

Turnaround Time

5 days

Test Code

9676

Specimen Type

• Tissue

Specimen Requirements

Tube/Container Type:

Preferred

• Slide(s)

Pre-testing Requirements

Only tissue preserved in neutral buffered formalin and paraffin embedded is suitable for use.

NB: Samples de-calcified in formic acid are not suitable for FISH as this process affects the integrity of the DNA
Tissue specimens should be cut into sections of 3-4µm, on poly-L-lysine slides.

The H&E slide must be assessed by a pathologist prior to receipt and appropriate areas for FISH analysis clearly marked.

Ensure one H&E slide and 5~8 unprocessed slides are supplied.

Slide must be clearly labelled with two patient identifiers, matching those on the request form.

Aliquot Instructions

Transport to Lab

Ambient (8 - 24 degrees Celsius)

Additional Referral/Aliquot Information

Transport in slide-holders or boxes, at ambient temperature

Interpretation

Clinically significant rearrangements and copy number changes outside of established normal ranges will be reported with relevant interpretive comment.

Test Method

Fluorescent in situ hybridisation (FISH) on formalin fixed paraffin embedded (FFPE) tissue.

Tests available:

MDM2 amplification
HER2 amplification
ROS1 rearrangement
ALK rearrangement
MYC Rearrangement
IGH/MYC gene fusion
BCL2 Rearrangement
BCL6 Rearrangement
IGH/BCL2 gene fusion

The same probes may be applied to a standard blood or bone marrow preparation if required. See 'Haematological and Oncological FISH'

Non-Funded Test Cost

$470.78 (Exclusive of GST)

Billing Code

9678

Additional Costing Information

The price listed is per probe set. Testing using multiple probes sets will be billed accordingly.

Contact Information

Enquiry Form