Quantitative PCR for BCR::ABL1 (p190) Fusion Transcript

Diagnostic Use

Quantitation of BCR::ABL1 p190 transcripts in peripheral blood and bone marrow of ALL and CML patients for diagnosis and monitoring using the Xpert BCR-ABL Ultra p190 assay (GeneXpert, Cepheid).

Department

Genetics - Molecular Oncology

Delphic Registration Code

MONC

Laboratory Handling

Phlebotomy

3x EDTA (Lavender) dedicated to Genetics

Registration

Send to MONC

Synonyms

BCR-ABL1 Quantitative p190 PCR
BCR-ABL1
p190
CML Molecular Monitoring
CML
ALL Molecular Monitoring
ALL

Turnaround Time

5 days

Specimen Type

• Whole Blood
• Bone Marrow

Specimen Requirements

Tube/Container Type:

Preferred

• EDTA (Lavender Cap)
• Please refer to Additional Information below

Additional Specimen Information

12 ml peripheral blood (EDTA)
2-4 ml bone marrow (EDTA, not RPMI)
Transport to the laboratory at ambient temperature within 72 hours of collection.

Aliquot Instructions

Sample Type

• Whole Blood

Transport to Lab

Ambient (8 - 24 degrees Celsius)

Additional Referral/Aliquot Information

Transport to the laboratory at ambient temperature within 72 hours of collection.

Interpretation

Transcripts from BCR::ABL1 e1a2 (p190) and an endogenous control gene ABL1 are amplified and results are expressed as a ratio percent (BCR::ABL1/ABL x 100).  The Lower Limit of Quantitation is 0.007%.
The significance of BCR::ABL1 transcript level should always be considered in the context of treatment and sequential BCR::ABL1 monitoring.

Please Note: This assay will only detect the BCR::ABL1 p190 transcript. If a diagnostic sample is negative for BCR-ABL1 p190 transcripts by the Xpert BCR::ABL Ultra Assay we recommend that BCR::ABL1 testing by FISH is performed to exclude other BCR::ABL1 transcripts associated with CML/ALL.

Non-Funded Test Cost

$432.01 (Exclusive of GST)

Billing Code

5046

Additional Costing Information

An additional charge of approximately $200 may be applied to samples received on weekends and public holidays for processing by on-call staff.
In the case of this test the cost will only be incurred if the sample will be older than 72 hours post collection by the next business day.

Contact Information

Enquiry Form

Contact Information

Enquiry Form