PNH test by Flow Cytometry, Blood

Department

Haematology - Surface Markers

Delphic Registration Code

SMBH

Synonyms

Flow cytometry PNH Screen
PNH Screen flow cytometry
Surface Marker PNH screen

Turnaround Time

3 days

Specimen Type

• Whole Blood

Specimen Requirements

Tube/Container Type:

Preferred

• EDTA (Lavender Cap)

Preferred Sample Volume (ml)

10

No of Samples Required

2

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• EDTA (Lavender Cap)

Preferred Sample Volume (ml)

5

Additional Specimen Information

Please indicate if the patient has received RBC transfusion within the previous three months.
Transport to lab room temperature within 48 hours.

Aliquot Instructions

Sample Type

• Whole Blood

Minimum Volume (ml)

5

Transport to Lab

Ambient (8 - 24 degrees Celsius)

Additional Referral/Aliquot Information

Minimum aliquot 1 x 5ml EDTA blood. Preferred aliquot 2 x 5ml EDTA blood. Bone Marrow not accepted. Samples must be stored at room temperature and delivered to the laboratory within 48 hours.

Interpretation

Granulocytes are tested for the GPI linked antigen CD24 and FLAER (fluorescent aerolysin – a marker for the GPI anchor). Monocytes are tested for the GPI linked antigen CD14 and FLAER. Red blood cells are tested for the the GPI linked antigen CD59.

Cells negative or weakly positive for GPI linked antigens and/or FLAER indicates the presence of a PNH clone.

Test Method

Flow Cytometry is the preferred method for PNH testing.

Non-Funded Test Cost

$377.49 (Exclusive of GST)

Billing Code

8272

Samples should be received by lab before 12 noon on Fridays.

Contact Information

Enquiry Form