Neutrophil Oxidative Burst Measurement

Department

Immunology

Delphic Registration Code

NEUS

Laboratory Handling

Registration

Phone lab and send direct to immunology or after hours place into immunology bin at room temperature

Synonyms

CGD
Chronic Granulomatous Disease
DHR
Dihydro Rhodamine
NBT assay
Nitroblue tetrazolium assay

Turnaround Time

2 days

Test Code

6979

Specimen Type

• Whole Blood

Specimen Requirements

Tube/Container Type:

Preferred

• EDTA (Lavender Cap)

Preferred Sample Volume (ml)

5

No of Samples Required

2

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• EDTA (Lavender Cap)

Preferred Sample Volume (ml)

2

Additional Specimen Information

ESSENTIAL:
Contact IMMUNOLOGY laboratory prior to collection.
Sample MUST arrive in Immunology CHL within 24 hours of collection
Patient MUST not have an ongoing infection at time of collection
Please send a CBC for both patient and control sample to allow evaluation of eosinophil numbers

Aliquot Instructions

Sample Type

• Whole Blood

Minimum Volume (ml)

2

Transport to Lab

Ambient (8 - 24 degrees Celsius)

Additional Referral/Aliquot Information

1 x 2 mL EDTA blood (Paeds) + control EDTA, 1 x 5 mL EDTA blood + control EDTA.
transport to lab at room temperature ASAP at within 24 hours of collection

Test Method

Flow cytometry using the fluorescent dye DHR (dihydrorhodamine 123).

Non-Funded Test Cost

$145.79 (Exclusive of GST)

Billing Code

6979

Additional Costing Information

BY PRIOR ARRANGEMENT ONLY - contact the laboratory before collection
A control specimen MUST be included with specimens being sent from outside Christchurch so that the effects of transport and storage can be ascertained.
Please NOTE if the laboratory is not given at least 24 hours notice that a sample is being sent then an urgent charge will be applied to this test.
This test is not offered as an urgent assay and will not be processed if received at the weekend.
This test replaces the NBT assay.

Contact Information

Enquiry Form