Neutrophil Oxidative Burst Measurement

Diagnostic Use

This method measures the ability of a patient’s neutrophils to produce superoxide and other reactive oxygen species following cell activation. Chronic granulomatosus disease (CGD) is a rare disorder caused by defects in the NADPH oxidase enzyme complex of phagocytes.

ESSENTIAL:
Contact IMMUNOLOGY laboratory prior to collection
Patient MUST NOT have an ongoing infection at time of collection
Sample MUST be tested within 24 hours of venipuncture
The sample MUST be received by the immunology laboratory between 8am - 2.30pm Monday to Thursday and by midday on Fridays
A control specimen MUST be included with specimens so that the effects of transport and storage can be ascertained.
Please NOTE if the laboratory is not given at least 24 hours notice that a sample is being sent then an urgent charge will be applied to this test.
This test is not offered as an urgent assay and will not be processed if received at the weekend.

Department

Immunology

Delphic Registration Code

NEUS

Synonyms

CGD
Chronic Granulomatous Disease
DHR
Dihydro Rhodamine
NBT assay
Nitroblue tetrazolium assay

Turnaround Time

2 days

Test Code

6979

Specimen Type

• Whole Blood

Specimen Requirements

Tube/Container Type:

Preferred

• EDTA (Lavender Cap)

Preferred Sample Volume (ml)

5

No of Samples Required

2

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• EDTA (Lavender Cap)

Preferred Sample Volume (ml)

2

Additional Specimen Information

ESSENTIAL:
Contact IMMUNOLOGY laboratory prior to collection
Patient MUST NOT have an ongoing infection at time of collection
Sample MUST be tested within 24 hours of venipuncture
The sample MUST be received by the immunology laboratory between 8am - 2.30pm Monday to Thursday and by midday on Fridays
A control specimen MUST be included with specimens so that the effects of transport and storage can be ascertained.
Please NOTE if the laboratory is not given at least 24 hours notice that a sample is being sent then an urgent charge will be applied to this test.
This test is not offered as an urgent assay and will not be processed if received at the weekend.
This test replaces the NBT assay.

Aliquot Instructions

Sample Type

• Whole Blood

Minimum Volume (ml)

2

Transport to Lab

Ambient (8 - 24 degrees Celsius)

Additional Referral/Aliquot Information

1 x 2 mL EDTA blood (Paeds) + control EDTA, 1 x 5 mL EDTA blood + control EDTA.
transport to lab at room temperature ASAP within 24 hours of collection

Interpretation

All abnormal findings will be reviewed by an Immunopathologist, where bespoke comment may be applicable.

Test Method

Flow cytometry using the fluorescent dye DHR (dihydrorhodamine 123).

Non-Funded Test Cost

$161.50 (Exclusive of GST)

Billing Code

6979

ESSENTIAL:
Contact IMMUNOLOGY laboratory prior to collection
Patient MUST NOT have an ongoing infection at time of collection
Sample MUST be tested within 24 hours of venipuncture
The sample MUST be received by the immunology laboratory between 8am - 2.30pm Monday to Thursday and by midday on Fridays
A control specimen MUST be included with specimens so that the effects of transport and storage can be ascertained.
Please NOTE if the laboratory is not given at least 24 hours notice that a sample is being sent then an urgent charge will be applied to this test.
This test is not offered as an urgent assay and will not be processed if received at the weekend.
This test replaces the NBT assay.

Contact Information

Enquiry Form