MYD88 PCR

Diagnostic Use

Test to determine if there is a L265P variant in the MYD88 gene.

Please notify the laboratory if this result is required urgently.

Department

Genetics - Molecular Oncology

Delphic Registration Code

MONC

Laboratory Handling

Phlebotomy

1x EDTA (Lavender) dedicated to Genetics

Registration

Send to MONC

Synonyms

MYD88 L265P PCR

Turnaround Time

14 days

Specimen Type

• Whole Blood
• Bone Marrow
• CSF

Specimen Requirements

Tube/Container Type:

Preferred

• EDTA (Lavender Cap)

Acceptable

• RPMI (Bone Marrow)

Additional Specimen Information

Bone marrow collected in EDTA or media. Bone marrow preferred specimen - at least 1 mL.
Peripheral blood (EDTA)/FFPET specimens can be used but may yield false negative results - a comment will be written on the report.
Send ambient to the laboratory.

Aliquot Instructions

Interpretation

The MYD88 L265P activating variant is common in several B-cell lymphoproliferative disorders including Waldenstrom’s macroglobulinemia (85%), lymphoplasmacytic lymphoma (85%), IgM MGUS (52%) and diffuse large B-cell lymphoma (DLBCL) occurring in immune privileged sites such as cerebral and leg-type lymphomas (60%) (PMID: 31699794).

Reference Intervals

Result reported as “Detected” or “Not detected”.

Test Method

Droplet digital PCR using the Bio-Rad PrimePCR ddPCR Mutation Detection Assay for NM_002468.4 (MYD88):c.794T>C (p.Leu265Pro).

Limit of Quantitation: 0.2% mutant copies over total MYD88 gene copies.

Non-Funded Test Cost

$261.25 (Exclusive of GST)

Billing Code

5040

Please notify the laboratory if this result is required urgently.

Contact Information

Enquiry Form