Test to determine if there is a L265P variant in the MYD88 gene.
Genetics - Molecular Oncology
1x EDTA (Lavender) dedicated to Genetics
Send to MONC
MYD88 L265P PCR
Bone marrow collected in EDTA or media. Bone marrow preferred specimen - at least 1 mL.
Peripheral blood (EDTA)/FFPET specimens can be used but may yield false negative results - a comment will be written on the report.
Send ambient to the laboratory.
The MYD88 L265P activating variant is common in several B-cell lymphoproliferative disorders including Waldenstrom’s macroglobulinemia (85%), lymphoplasmacytic lymphoma (85%), IgM MGUS (52%) and diffuse large B-cell lymphoma (DLBCL) occurring in immune privileged sites such as cerebral and leg-type lymphomas (60%) (PMID: 31699794).
Result reported as “Detected” or “Not detected”.
Droplet digital PCR using the Bio-Rad PrimePCR ddPCR Mutation Detection Assay for NM_002468.4 (MYD88):c.794T>C (p.Leu265Pro).
Limit of Quantitation: 0.2% mutant copies over total MYD88 gene copies.