Microbiology - Bacteriology
Acid Fast Bacilli stain
TB stain and culture
Notify laboratory immediately if results are required urgently. Please phone (03) 3640300 Ext 81356 or Ext 88352.
ALL samples are to be well labelled with patient details, NHI and/or DOB and dates of collection.
Sample: sputum (3 consecutive days), bronchial washings, faeces, tissue, aspirates, CSF, pleural fluid, scope QCs, blood and bone marrow.
For blood or bone marrow TB testing, request special media from Bacteriology lab on Ext 80117.
Urine is not a suitable specimen to exclude pulmonary Tb, please only send Early Morning Urine, 50ml, to exclude renal/systemic Tb (over 3 consecutive days)
Sample Transportation: Gastric aspirates must be neutralised prior to sending if transit time is > 4hrs. Please contact lab prior to sending.
Ambient (8 - 24 degrees Celsius)
For treatment of non-tuberculous Mycobacterium refer to the link below.
Diagnosis, Treatment and Prevention of Nontuberculous Mycobacterial Disease.
Microscopy by way of Auramine and ZN. Culture solid media and broth continuous monitoring. Direct PCR is available on request.
Direct PCR by GeneXpert for Mycobacterium tuberculosis (MTB) and Rifampicin susceptibility.
PCR by Seegene AnyPlex for MTB/non-tuberculous Mycobacteria only after discussion with Microbiologist.
Genotypic susceptibilities for Rifampicin, Isoniazid and Pyrazinamide.
Phenotypic susceptibilities for Rifampicin, Isoniazid, Ethambutol and Pyrazinamide. Moxifloxacin on request.
Susceptibility testing for rapid growers available.
Tb DNA amplication test is available on request.
PCR is available on paraffin embedded tissues after consultation with the laboratory.
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