High-grade B-cell Lymphomas were redefined into two distinct categories in the 2017 WHO classification of Tumours and Haematopoietic and Lymphoid Tissues.
The first category, High-grade B-cell lymphoma with MYC and BCL2 and/or BCL6 rearrangements, encompasses all B-cell lymphomas that have MYC rearrangement in combination with a BCL2 and/or BCL6 rearrangement.
The second category, High-grade B cell lymphoma, NOS, lack the double or triple hit.
Genetics - Cytogenetics
BCL2 FFPE FISH testing
BCL6 FFPE FISH testing
FISH test- MYC,IGH,BCL2,BCL6
FISH testing - Lymphoma (FFPE)
IGH FFPE FISH testing
MYC FFPE FISH testing
Only tissue preserved in neutral buffered formalin and paraffin embedded is suitable for use.
NB: Samples de-calcified in formic acid are not suitable for FISH as this process affects the integrity of the DNA
Tissue specimens should be cut into sections of 2-4µm, on poly-L-lysine slides.
The H&E slide must be assessed by a pathologist prior to receipt and appropriate areas for FISH analysis clearly marked.
Ensure one H&E slide and 5~8 unprocessed slides are supplied.
Slide must be clearly labelled with two patient identifiers, matching those on the request form.
Ambient (8 - 24 degrees Celsius)
Transport in slide-holders or boxes, at ambient temperature
Clinically significant rearrangements and copy number changes outside of established normal ranges will be reported with relevant interpretive comment.
Fluorescent in situ hybridisation (FISH) on formalin fixed paraffin embedded (FFPE) tissue.
IGH/MYC gene fusion
IGH/BCL2 gene fusion
The same probes may be applied to a standard blood or bone marrow preparation if required. See 'Haematological and Oncological FISH'
$444.13 (Exclusive of GST)
The price listed is per probe set. Testing using multiple probes sets will be billed accordingly.
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