Test used for the detection or exlcusion of clonal B cell populations.
Genetics - Molecular Oncology
3x EDTA (Lavender) dedicated to Genetics
Send to MONC
B-cell gene rearrangement PCR
IgH gene rearrangement PCR
12 ml peripheral blood in EDTA. 1-3 ml of bone marrow in EDTA or RPMI (anticoagulant).
5 mm cube of fresh (in saline or RPMI) or frozen tissue.
FFPET - block or at least 10 x 10 micron slices
Clonal populations of cells yield one (monoclonal), two (clonal), or more (oligoclonal) prominent amplicons within a specified size range.
A normal (polyclonal) population of B cells will produce a polyclonal smear representing a heterogeneous population of V-J region rearrangements and, if graphed, would resemble a bell-shaped Gaussian curve.
IGH PCR amplification is carried out using primers homologous to sequences of all three conserved framework regions (FR1, FR2 and FR3), and the joining (J) region of the immunoglobulin heavy chain (IGH) gene.
Sensitivity approximately 2%.
Paraffin-embedded tissue may, or may not, yield DNA of a suitable quality or quantity for this assay and may be reported as Insufficient.
Contact Canterbury Health Laboratories on +64 3 364 0484 or email LabInfo@cdhb.health.nz
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