HLA-B27

Diagnostic Use

Ankylosing spondylitis (AS) is a chronic inflammatory disease affecting the musculoskeletal system and characterised by absence of ANA and rheumatoid factor. The association between AS and HLA-B27 is so pronounced that the presence of B27 can be used as a diagnostic aid very early in disease development. 90-95% of AS patients are HLA-B27 positive compared with only 5-8% of the normal (Caucasian) population.

Department

Genetics - Molecular Pathology

Delphic Registration Code

B27

Synonyms

ankylosing spondylitis.
B27 genotyping

Turnaround Time

4 weeks

Test Code

6992

Specimen Type

• Whole Blood

Specimen Requirements

Tube/Container Type:

Preferred

• EDTA (Lavender Cap)

Preferred Sample Volume (ml)

4.0

No of Samples Required

1

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• EDTA (Lavender Cap)

Preferred Sample Volume (ml)

1.0

Minimum Sample Volume (ml)

0.5

Additional Specimen Information

Deliver to lab as soon as practicable (may be refrigerated overnight or up to 72 hours before sending)
Clotted samples unacceptable.

Aliquot Instructions

Sample Type

• Whole Blood

Additional Referral/Aliquot Information

Deliver to lab as soon as practicable (may be refrigerated overnight or up to 72 hours before sending)

Test Method

The assay uses a real-time PCR method for the detection of the HLA-B27 genotype using allele-specific primers combined with a TaqMan fluorescent minor groove binder (MGB) probe.

Non-Funded Test Cost

$64.06 (Exclusive of GST)

Billing Code

6992

Contact Information

Enquiry Form