Performed as part of a constituent of tests to identify abnormal haemoglobins.
Haematology - Special Tests
Haemoglobin alkaline electrophoresis
Hb electrophoresis - alkaline
No blood transfusion or iron treatment in the preceeding 3 months.
Ambient (8 - 24 degrees Celsius)
If overnight - Chilled (2 - 8 degrees Celsius)
Preferred specimen - 2 x 4.0mL EDTA whole blood, minimum aliquot 2mL EDTA whole blood
Haemoglobin carries a positive charge when in an environment on the acidic side of its iso-electric point (pH 6.8) and a negative charge when in an environment on the alkaline side of its iso-electric point. In an alkaline buffer, the haemoglobin assumes a negative charge and migrates towards the anode. Each type of haemoglobin has a different concentration of negative charges and separates into different bands during electrophoresis; the migratory position of any abnormal band/s present is compared with the control sample.
Hb electrophoresis at alkaline pH
$68.92 (Exclusive of GST)
Forms part of a haemoglobinopathy/thalassaemia screen. Not performed as a standalone test.
Test Availability: Mon - Fri, 0800 - 1700
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