Performed as part of a constituent of tests to identify abnormal haemoglobins.
Haematology - Special Tests
No blood transfusion or iron treatment in the preceeding 3 months.
Ambient (8 - 24 degrees Celsius)
If overnight - Chilled (2 - 8 degrees Celsius)
Preferred aliquot 2 x 4.0mL EDTA whole blood, minimum aliquot 2mL EDTA whole blood
Acid gel electrophoresis is used to further differentiate haemoglobins with electrophoretically similar migration points at alkaline pH. Haemoglobin will carry a positive charge when in an environment on the acidic side of its iso-electric point (pH 6.8) and will migrate toward the cathode (negative electrode). The position of the abnormal band on alkaline electrophoresis is compared with its migratory position on acid electrophoresis; this will differentiate Hb C from Hb E and Hb S from Hb D.
SEBIA hydragel acid electrophoresis
$68.02 (Exclusive of GST)