FISH, Haematological/Oncological

Diagnostic Use

Available tests include (but not limited to):
- ATM deletion
- BCR/ABL1 gene fusion
- CBFB gene rearrangement
- D20S108 (20q) deletion
- ETV6/RUNX1 gene fusion
- FGFR1 gene rearrangement
- IGH gene rearrangement
- IGH/BCL2 gene fusion
- IGH/CCND1 gene fusion
- IGH/FGFR3 gene fusion
- IGH/MAF gene fusion
- IGH/MAFB gene fusion
- IGH/MYC gene fusion
- JAK2 gene rearrangement
- KMT2A(MLL) gene rearrangement
- MECOM rearrangement
- MYC gene rearrangement
- MYCN gene enumeration/amplification
- PDGFRA gene rearrangement
- PDGFRB gene rearrangement
- PML/RARA gene fusion
- RUNX1/RUNX1T1 gene fusion
- TP53 deletion (for gene mutation testing see 'Tumour-15 NGS panel')
- 13q14.3 deletion
- Various chromosome enumeration

Current FISH Panels

Paediatric ALL Panel:
- 4 & 10 enumeration (Double Trisomy)
- BCR/ABL1 gene fusion
- ETV6/RUNX1 gene fusion
- KMT2A(MLL) gene rearrangement

Myeloma panel:

- Chromosomes 1 abnormalities (1p deletion/1q gain)
- TP53 deletion
- IGH gene rearrangement
- IGH cascade if indicated with no high-risk findings (sequentially; IGH/CCND1, IGH/FGFR3, IGH/MAF, IGH/MAFB)

Eosinophilia panel:
- PDGFRA gene rearrangement
- PDGFRB gene rearrangement
- FGFR1 gene rearrangement
- JAK2 gene rearrangement (upon specific request)
- BCR/ABL1 gene fusion (upon specific request)

Department

Genetics - Cytogenetics

Delphic Registration Code

CTGN

Laboratory Handling

Phlebotomy

Please ensure a separate tube is taken for this test. Requests for "mutation" testing are not for FISH and require EDTA blood.

Registration

Requests for "mutation" testing are for Molecular Pathology and require EDTA blood.

Test Adds

Please note that requests for "mutation" or "gene" or "variant" testing are for Molecular Pathology and require EDTA blood.

Synonyms

ALL FISH panel
CLL FISH panel
FISH - 20q12 Deletion Probe
FISH - BCR/ABL1 Rearrangements
FISH - CBFB, inv(16) Rearrange
FISH - ETV6/RUNX1(TEL/AML1) Tr
FISH - MLL Rearrangements
FISH - MYC Rearrangements
FISH, Haematological
Lymphoma FISH panel
Multiple Myeloma FISH panel

Turnaround Time

2 weeks

Specimen Type

• Whole Blood
• Bone Marrow
• Tissue

Specimen Requirements

Tube/Container Type:

Preferred

• Lithium Heparin - Non-gel (Green Cap)

Acceptable

• RPMI (Bone Marrow)
• RPMI (Tissue)
• Slide(s)

Additional Specimen Information

Send all specimens without delay at ambient temperature. Do not refrigerate, freeze or centrifuge.
Transport medium is available from the laboratory by contacting LabInfo@cdhb.health.nz

Tissue

Bone Marrow •>1ml bone marrow aspirate in transport medium
•Bone marrow aspirate smear slide (2x slides)

Peripheral Blood •At diagnosis: 5-10mL Lithium Heparin (green top)
•Minimal residual disease: 10mL Lithium Heparin (green top)
Note: Whole blood is required for genetic testing; please DO NOT centrifuge blood tubes

Tumour/Lymph Node >1cm tumour/lymph node tissue in transport medium
Tumour touch preparation slide (2x slides)

Aliquot Instructions

Transport to Lab

Ambient (8 - 24 degrees Celsius)

Additional Referral/Aliquot Information

DO NOT refrigerate, freeze or centrifuge

Reference Intervals

Normal and abnormal ranges have been established in-house as part of the validation process for each probe. Please contact the laboratory for further details.

Test Method

Fluorescence In Situ Hybridisation (FISH) is a molecular cytogenetic technique that can be described as a’ hybrid’ of molecular genetic and conventional cytogenetic technologies. The technique combines the ability to identify a specific gene or gene region with direct visualisation of cells and/or chromosomes under the microscope.

FISH is usually applied to standard cytogenetic preparations, on glass slides and both cultured and uncultured cells can be used, unlike standard cytogenetic techniques which require actively-dividing cells. This means that a variety of specimen types can be processed successfully, even those that conventionally don’t grow well in culture, making FISH a powerful tool for the study of acquired abnormality in bone marrows, tumours and even paraffin-embedded specimens. As a complementary technique, or as a test for targeted abnormalities, FISH has expanded our capabilities for more accurate, rapid and refined cytogenetic diagnoses.

Non-Funded Test Cost

$336.17 (Exclusive of GST)

Billing Code

9483

Additional Costing Information

An additional charge for direct interphase preparation applies where cell culture, harvest and hold or chromosome analysis has not also been requested.

Urgent turnaround time 1-3 days by arrangement.

Uncertainty of Measurement Please contact the laboratory if you have specific queries regarding our testing.

Contact Information

Enquiry Form