Fatty Acids - free
Fatty Acids - non esterified
Non-Esterified Fatty Acids
Fasting sample preferred
Specimens must be collected on ice and the plasma separated from the red cells within 30 minute of collection and immediately frozen.
If overnight - Frozen
1 mL plasma store frozen.Minimum 250 uL plasma. Haemolysed samples unacceptable.
Enzymatic determination.acyl-CoA synthatase substrate.
$63.46 (Exclusive of GST)
EDTA anti-coagulated blood collected on ice is the preferred sample - delays in the processing of the sample leads to an increase in free fatty acid levels due to lipolysis of the triglycerides by lipase. If serum is used the clotting of the sample is delayed and lipolysis occurs leading to higher free fatty acid levels. haemolysed samples are also unacceptable