Microbiology - Virology
Full clinical details are essential.
Tissue consult lab
Ambient (8 - 24 degrees Celsius)
Post-transplant Lymphoproliferative disorder (PTLD)
PCR assays performed on PBLs are positive in only 10-20% of normal EBV-seropositive patients and quantity of EBV DNA is low. Most patients with PTLD have high levels of EBV DNA that can be distinguished by quantitation. Some PTLD patients do not have elevated levels of EBV DNA and some transplant recipients with elevated levels in the same range as PTLD do not develop clinically evident PTLD.
It has not been clearly established whether EBV DNA may be detected in CSF during uncomplicated infectious mononucleosis, in other neurological illnesses, or even apart form any illness. The recommended approach is to use serological diagnosis to determine acute infection combined with CSF PCR to provide evidence of CNS involvement.
Dissociated CNS lymphoma: EBV PCR may be helpful in distinguishing lymphoma from other syndromes. A negative result does not rule out lymphoma.
EBV DNA detection using nucleic acid amplification techniques.
$203.99 (Exclusive of GST)
Serological investigations are recommended prior to requesting DNA testing.
Diagnosis of EBV infection in immunocompromised patients can be problematic. Serological testing may be difficult to interpret due to most adults being seropositive due to prior EBV infections.
Post-transplant lymphoproliferative disorder (PTLD) is the disease most commonly associated with immunocompromised patients. It causes abnormal proliferation of lymphoid tissue which can occur after solid organ or bone marrow transplantation.
EBV is known to cause encephalitis and other neurological syndromes. EBV is associated with most cases of primary CNS lymphoma in patients with AIDS.
Infectious mononucleosis (Glandular Fever)
Diagnosis by serological tests is recommended. Refer:
Paul Bunnell or Mono-spot test (heterophile antibody test)
Specific antibody assays - Epstein Barr virus early ag-Ab, Epstein Barr virus nuclear ag-Ab, Epstein Barr virus VCA IgG-Ab, Epstein Barr virus VCA IgM-Ab.