Human enteroviruses are ubiquitous picornaviruses found throughout the world and are transmitted from person to person through fecal-oral contact and, less commonly, respiratory secretions.
Polioviruses, the prototypic enteroviruses, are the cause of paralytic poliomyelitis, a disease that has been eradicated in most high-income countries and targeted for worldwide eradication. The non-polio enteroviruses (comprising the group A and B coxsackieviruses, echoviruses, and “newer” enteroviruses) and the parechoviruses are responsible for a wide spectrum of disease in persons of all ages, although infection and illness disproportionately affect infants and young children.
More than 90 percent of infections caused by the non-polio enteroviruses are asymptomatic or result only in an undifferentiated febrile illness. When more serious disease occurs, the clinical spectrum and disease severity vary with the age, sex, and immune status of the host. Some clinical syndromes (viral meningitis and some exanthema) are caused by numerous enterovirus serotypes, while others appear limited to specific enterovirus subgroups (eg, hand, foot, and mouth disease [HFMD] with enterovirus A71 and some group A coxsackieviruses, and pleurodynia and myocarditis with the group B coxsackieviruses).
Samples that meet testing criteria are referred to ESR for typing.
Microbiology - Virology
For CSF samples register CNSP; for respiratory requests register IRNA
Testing performed routinely Monday-Friday
Enterovirus eye swab
Enterovirus throat swab
Hand foot and mouth
Chilled (2 - 8 degrees Celsius)
This assay is used for the detection of Enteroviral RNA in a variety of non-CSF samples from patients with enteroviral infections. RNA detection by PCR provides a rapid and definitive diagnosis for the presence of Enteroviral nucleic acid.
This assay is known to detect Poliovirus 1,2,3. Coxsackie A: A1-22, A24. Coxsackie B: B1-6. Echovirus 1-9, 11-27, 29-33. Enterovirus 68-70.
Positive samples are forwarded to ESR for subtyping
If Faecal and throat swab samples are submitted following the detection of Enteroviral RNA in a CSF sample, those samples are not tested at CH Labs but forwarded directly to ESR for Enterovirus PCR and subtyping. No charge is made for testing these samples.
Results are reported as Enterovirus RNA DETECTED or Enterovirus RNA NOT detected.
PCR results should always be interpreted in conjunction with clinical history or symptomatology
Nucleic acid extraction and RT-PCR for Enterovirus RNA
$84.29 (Exclusive of GST)
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