DAT, Blood

Diagnostic Use

A Direct Antiglobulin Test is useful in demonstrating in vivo coating of red blood cells with IgG or the complement component C3d in the following settings:
-Autoimmune hemolytic anemia
-Hemolytic transfusion reactions
-Drug-induced hemolytic anemia

Department

NZBS Christchurch

Delphic Registration Code

TMC

Synonyms

Coombs test
DCT
Direct antiglobulin test
Direct Coombs test

Turnaround Time

72 hours

Test Code

5354

Specimen Type

• Whole Blood

Specimen Requirements

Tube/Container Type:

Preferred

• Cross Match (Pink Cap)

Preferred Sample Volume (ml)

6.0

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• EDTA (Lavender Cap)

Additional Specimen Information

Paediatric Specimen - Cord sample or heel prick EDTA blood

Aliquot Instructions

Transport to Lab

Chilled (2 - 8 degrees Celsius)
If overnight - Chilled (2 - 8 degrees Celsius)

Additional Referral/Aliquot Information

Aliquot Instructions - preferred aliquot 1 x 6.0mL pink top (EDTA) minimum aliquot 1mL EDTA whole blood

Interpretation

Negative: No IgG antibody or complement (C3d) detected on the surface of the red cell.

Positive: Test polyspecific direct antiglobulin testing will be ordered and performed.

Reference Intervals

Negative

Test Method

Bio-Rad microtyping system

Non-Funded Test Cost

$25.06 (Exclusive of GST)

This test is performed by New Zealand Blood Service, Blood Bank Christchurch Hospital. Ph: 03 364 0310,Fax: 03 364 0159

Contact Information

Enquiry Form