Chromosome analysis (karyotype), Amniotic Fluid

Diagnostic Use

Detection of microscopically visible unbalanced and balanced chromosome changes. Resolution >10Mb.
eg.
• suspected chromosome aneuploidy eg. monosomy X (Turner syndrome), trisomy including mosaicism,
• confirmation of abnormal rapid aneuploidy screen,
• detection of known parentally-derived chromosome rearrangement previously identified by G-banded karyotype

Department

Genetics - Cytogenetics

Delphic Registration Code

CTGN

Synonyms

Amniocentesis, chromosomes
Karyotype, amniotic fluid
Prenatal cytogenetics, AF - chromosomes
Prenatal diagnosis, AF - chromosomes

Turnaround Time

15 days

Test Code

6781

Specimen Type

• Fluid

Specimen Requirements

Tube/Container Type:

Preferred

• Pottle

Preferred Sample Volume (ml)

20

Additional Specimen Information

Pre-Testing Requirements; Appropriate counselling by LMC or obstetrician.
Specimen Collection Protocols Approximately 15-20mL of amniotic fluid - sterile, clearly labelled with patient details. If fluid appears blood-stained, place the first few blood-stained mls into one universal and the remainder into a second.
Send to laboratory in "Prenatal Transport" pack as soon as possible.
"Prenatal Transport" packs are available by phoning the Cytogenetics laboratory.

Aliquot Instructions

Transport to Lab

Ambient (8 - 24 degrees Celsius)

Additional Referral/Aliquot Information

Please notify us on dispatch by phone or email.

Analysis and interpretation of this assay requires knowledge of the biological sex of the patient being tested. Please assist us in delivering quality results by providing accurate biological sex information on all referrals for this testing.

Interpretation

Comment on report

Reference Intervals

Comment on report

Test Method

Fetal cells (amniocytes) are cultured in growth medium for around 8-14 days and then harvested at the metaphase stage of the cell cycle when chromosomes are visible by light microscopy. Slide preparations are treated with trypsin and then stained (G-banding). The G-banded chromosome preparations are analysed using light microscopy (karyotyping). Routine analysis comprises of 5 metaphases fully analysed and 10 counted.

Non-Funded Test Cost

$690.27 (Exclusive of GST)

Billing Code

6781

Additional Costing Information

Samples must be sterile. Occasionally, poor cell growth will result in a delayed result. This is more frequent with heavily blood-stained, or small samples.
All prenatal requests are treated as urgent.

Contact Information

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