Chimerism Analysis

Diagnostic Use

Chimerism analysis determines percent donor/recipient cells in the peripheral blood or bone marrow following a bone marrow transplant.
Note that T cells and neutrophils are initially isolated from peripheral blood for separate chimerism analyses.

Please contact the laboratory (genelab.test@cdhb.health.nz, ext 86008) before collecting samples from children for chimerism analysis requests.

Department

Genetics - Molecular Oncology

Delphic Registration Code

MONC

Laboratory Handling

Phlebotomy

4x EDTA (Lavender) dedicated to Genetics

Registration

Please send samples directly to MONC

Turnaround Time

7 days

Test Code

7903

Specimen Type

• Whole Blood
• Bone Marrow

Specimen Requirements

Tube/Container Type:

Preferred

• EDTA (Lavender Cap)

Acceptable

• RPMI (Bone Marrow)

Additional Specimen Information

Please either coordinate with the Bone Marrow Transplant Coordinators (BMTcoordinators@cdhb.health.nz) or the Molecular Oncology team (genelab.test@cdhb.health.nz, ext 86008).
Please contact the laboratory (genelab.test@cdhb.health.nz, ext 86008) before collecting samples from children for chimerism analysis requests.

Note: Donor and pre-transplant recipient (patient) DNA samples should be tested for informative alleles prior to chimerism analysis. These prior tests incur an additional cost for DNA extraction and informative allele screens by STR analysis. If a pre-transplant sample for the recipient is not available, it may be possible to determine pre-transplant informative alleles using DNA extracted from a buccal swab.

12 ml peripheral blood in EDTA. 1-3 ml bone marrow in EDTA or RPMI (with anticoagulant).
Transport to laboratory at ambient temperature within 24 hours of collection.

Aliquot Instructions

Test Method

The AmpFlSTR PCR Amplification Kit is a short tandem repeat (STR) multiplex assay that amplifies 15 tetranucleotide repeat loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA) and the Amelogenin gender-determining marker in a single PCR amplification.
Short tandem repeats (STR) of 2-4 bp in genomic DNA are often highly variable with regard to the number of repeats present and can be used to distinguish the DNA of one person from that of another. They are useful for monitoring the success of bone marrow transplants by showing whether the cells present in blood or bone marrow are those of the donor or recipient.

Non-Funded Test Cost

$849.16 (Exclusive of GST)

Billing Code

7903

Contact Information

Enquiry Form