cDNA is synthesised from RNA and then used as a template in reverse transcriptase (RT-PCR).
Department
Genetics - Molecular Oncology
Delphic Registration Code
MONC
Turnaround Time
7 days
Test Code
6955
Specimen Type
Whole Blood
Bone Marrow
Tissue
Specimen Requirements
Tube/Container Type:
Preferred
EDTA (Lavender Cap)
Acceptable
Pottle
RPMI (Bone Marrow)
Additional Specimen Information
Transport to the laboratory at ambient temperature within 48 hours of collection to ensure high-quality RNA can be extracted.
Aliquot Instructions
Test Method
RNA is extracted from cells and a complementary DNA (cDNA) template for PCR is synthesised from total RNA by reverse transcription using the SuperScript™ III First-Strand Synthesis System.
Non-Funded Test Cost
$212.82 (Exclusive of GST)
Billing Code
6955
Additional Costing Information
An additional charge of approximately $200 may be applied to samples received on weekends and public holidays for extraction of RNA by on-call staff.
