CD34 Flow Cytometry

Diagnostic Use

Flow cytometry analysis is used to enumerate the number of CD34+ Haematopoietic Stem Cells (HSCs) for patients undergoing stem cell transplantation. HSCs are measured in the peripheral blood to indicate the best time to perform the harvest collection. HSCs are measured again in the harvest product to ensure adequate numbers have been collected to go ahead with the transplant.

Department

Haematology - Surface Markers

Delphic Registration Code

SMBH

Turnaround Time

1 day

Test Code

1384

Specimen Type

• Whole Blood

Specimen Requirements

Tube/Container Type:

Preferred

• EDTA (Lavender Cap)

Preferred Sample Volume (ml)

5

No of Samples Required

1

Additional Specimen Information

EDTA peripheral blood; or Stem Cell Harvest (SCH) product (fresh or frozen) - approximately 0.5mL delivered by courier via NZBS

Aliquot Instructions

Transport to Lab

Ambient (8 - 24 degrees Celsius)
Frozen

Additional Referral/Aliquot Information

EDTA peripheral blood; or Stem Cell Harvest (SCH) product (fresh or frozen) - approximately 0.5mL delivered by courier via NZBS

Test Method

Flow cytometry analysis, single platform method.

Non-Funded Test Cost

$291.85 (Exclusive of GST)

Billing Code

1494

Turnaround time for phoned results is 4 hours, turnaround time for final authorised result is 1 day.

Uncertainty of Measurement
Determined in-house using CD-Chex CD34:
Absolute CD34: +/- 6.7% at 29.7 cells/uL (29.7+/- 2.0 cells/uL)
CD34 %: +/- 6.5% at 0.46% (0.46% +/- 0.03%)
WBC = +/- 3.7% at 6.5 x 10(9)/L (6.5 x 10(9)/L +/- 0.24 x 10(9)L)

Urgent testing by arrangement

Contact Information

Enquiry Form