Anti-nuclear antibodies (ANA) – titre

Department

Immunology

Delphic Registration Code

ANA

Synonyms

ANA - titre

Turnaround Time

14 days

Test Code

6940

Specimen Type

• Serum

Specimen Requirements

Tube/Container Type:

Preferred

• Serum (Red Cap)

Preferred if West Coast

• SST II (Gold Cap)

Acceptable

• SST II (Gold Cap)

Preferred Sample Volume (ml)

5

No of Samples Required

1

Paediatric Specimen Requirements

Tube/Container Type:

Preferred

• Serum (Red Cap)

Acceptable

• SST II (Gold Cap)

Preferred Sample Volume (ml)

0.5

Additional Specimen Information

Serum is required.
EDTA and Lithium heparin plasma has not been validated by the slide manufacturer.
A comment to this effect will accompany the results if plasma has been tested.

Aliquot Instructions

Sample Type

• Serum

Minimum Volume (ml)

0.1

Transport to Lab

Ambient (8 - 24 degrees Celsius)

Additional Referral/Aliquot Information

100 uL serum - minimum, 1 mL serum - preferred. Store in fridge

Reference Intervals

Systemic autoimmune disease is usually associated with titres greater than 1/80

Test Method

Indirect Immunofluorescence on Hep2000 cells. Titred from 1 in 160.

Non-Funded Test Cost

$37.75 (Exclusive of GST)

Billing Code

6940

Additional Costing Information

Anti-nuclear Antibodies - titre
Negative ANA usually rules out (99% sensitive using the 1982 ACR Classification Criteria) a diagnosis of SLE although anti Ro positive SLE patients may be negative. See comments on result reports regarding ANA patterns.
Positive ANA screens are always titred.

Contact Information

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