ALK is the abbreviated term for anaplastic large-cell lymphoma kinase, which is part of the Insulin receptor superfamily. ALK rearrangements occur in 2-7% of patients and these people respond to treatment with crizotinib, which has been FDA approved. The most common aberrant fusion gene resulting from an ALK rearrangement is the EML4-ALK fusion gene, but the ALK break-apart probe will detect all rearrangements of ALK.
Genetics - Cytogenetics
Formalin Fixed Paraffin Embedded Tissue section (2–4 microns thick) should be placed on positively -charged, poly-l-lysine coated slides.
Samples de-calcified in Formic acid are not suitable for FISH as this process affects the integrity of the DNA.
Specimen Collection Protocols:
•Ensure one H&E slide and 2-3 unprocessed slides are supplied for FFPE Tissue FISH. All slides must be clearly labelled with two patient identifiers identical to the request form.
•The H and E slide must be assessed by a pathologist prior to receipt, and appropriate areas for FISH analysis should be clearly marked.
Ambient (8 - 24 degrees Celsius)
Transport to lab in slide-holders or boxes at room temperature.
ALK rearrangement is reported if more than 15% of cells examined show a signal pattern consistent with a clinically significant ALK rearrangement.
Specimens are analysed and reported in accordance with CAP/ASCO recommendations (2013).
Rearrangements and copy number changes which have no proven clinical impact may also be detected using this probe. These will not be reported.
Fluorescence in situ hybridisation (FISH) on formalin fixed paraffin embedded (FFPE) tissue slides using a breakapart probe specific for the ALK rearrangement.
$444.13 (Exclusive of GST)
Please call the laboratory in advance if there is particular urgency for a result.
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