FISH can be applied to both cultured and uncultured cells unlike standard cytogenetic techniques which require actively-dividing cells.
This means that a variety of specimen types can be processed successfully, even those that conventionally don’t grow well in culture, making FISH a powerful tool for the study of acquired abnormality in bone marrows, tumours and even paraffin-embedded specimens.
FISH can determine the copy number of a region of interest and detect specific chromosome and/or and gene rearrangements involving that locus.
As a complementary technique, or as a test for targeted abnormalities, FISH has expanded our capabilities for more accurate, rapid and refined cytogenetic diagnoses.
Genetics - Cytogenetics
8q24 (MYC), Rearrangement - FISH
Burkitts Lymphoma - FISH
FISH, MYC rearrangement
t(8;14)(q24;q32) and Variants - FISH
Type of Specimen
Bone marrow 1mL bone marrow aspirate in transport medium.
Bone marrow smear slide - send at least two slides
Peripheral blood At diagnosis - 5-10 mL Lithium Heparin
Minimal residual disease - 10mL Lithium Heparin
Note: Whole blood is required for genetic testing; please do not centrifuge blood tubes.
Tumour At least 1 cm tumour tissue in transport medium
Tumour touch preparation slide - send at least two slides
Lymph node At least 1cm lymph node tissue in transport medium
Transport medium is available from the laboratory by contacting LabInfo@cdhb.health.nz
Ambient (8 - 24 degrees Celsius)
Do not refrigerate or freeze.
Comment on report.
Comment on report.
Fluorescence In Situ Hybridisation (FISH) can be described as a’ hybrid’ of molecular genetic and conventional cytogenetic technologies. Fluorescently-labelled DNA probes specific to a gene or region of interest are applied and hybridised to standard cytogenetic preparations on glass slides.
Probe signals are visualised on nuclei or chromosomes 'in situ' by fluorescent microscopy.
$809.05 (Exclusive of GST)
Urgent testing by arrangement. Urgent turnaround time 1-3 days