Fluorescence In Situ Hybridisation (FISH) is a targeted technique, described as a’ hybrid’ of molecular genetic and conventional cytogenetic technologies. FISH can be applied to both cultured and uncultured cells unlike standard cytogenetic techniques which require actively-dividing cells. This means that a variety of specimen types can be processed successfully, even those that conventionally don’t grow well in culture, making FISH a powerful tool for the study of acquired abnormality in bone marrows, tumours and paraffin-embedded specimens.
FISH can determine the copy number of a region of interest and detect specific chromosome and/or and gene rearrangements involving that locus. As a complementary technique, or as a test for targeted abnormalities, FISH has expanded our capabilities for more accurate, rapid and refined cytogenetic diagnoses.
Genetics - Cytogenetics
CTGN
ISH, ETV6/RUNX1 rearrangement
FISH, TEL/AML1 rearrangement
t(12;21)(p13;q22) - FISH
TEL/AML1 - FISH (TEL/AML1 is now referred to as ETV6/RUNX1)
18 days
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