Fluorescence In Situ Hybridisation (FISH) is a targeted technique, described as a’ hybrid’ of molecular genetic and conventional cytogenetic technologies. FISH can be applied to both cultured and uncultured cells unlike standard cytogenetic techniques which require actively-dividing cells. This means that a variety of specimen types can be processed successfully, even those that conventionally don’t grow well in culture, making FISH a powerful tool for the study of acquired abnormality in bone marrows, tumours and paraffin-embedded specimens.
FISH can determine the copy number of a region of interest and detect specific chromosome and/or and gene rearrangements involving that locus. As a complementary technique, or as a test for targeted abnormalities, FISH has expanded our capabilities for more accurate, rapid and refined cytogenetic diagnoses.
Genetics - Cytogenetics
CBFB - FISH
FISH, CBFB inversion 16
inv(16) and variants - FISH
Bone marrow 1mL bone marrow aspirate in transport medium.
Bone marrow smear slide - send at least two slides
Peripheral blood At diagnosis - 5-10 mL Lithium Heparin
Minimal residual disease - 10mL Lithium Heparin
Note: Whole blood is required for genetic testing; please DO NOT centrifuge blood tubes.
Transport medium is available from the laboratory by contacting LabInfo@cdhb.health.nz
Ambient (8 - 24 degrees Celsius)
Comment on report
Comment on report
Fluorescently-labelled DNA probes specific to a gene or region of interest are applied and hybridised to standard cytogenetic preparations on glass slides. Probe signals are visualised on nuclei or chromosomes 'in situ' by fluorescent microscopy.
$809.05 (Exclusive of GST)
Urgent testing by arrangement. Urgent turnaround time 1-3 days